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KMID : 0368420000430010048
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Journal of Plant Biology
2000 Volume.43 No. 1 p.48 ~ p.55
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Differential Expression of the Potato Proteinase Inhibitor Ⅱ Promoter in Transgenic Tobacco
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Kim, Su Jin
Lee, Mi-Yeon/An, Gyn Heung/Kim, Seung-Ryong
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Abstract
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We studied temporal and spatial expression patterns of the potato proteinase inhibitor Ⅱ (PI-Ⅱ) promoter, using trans-genie tobacco (Nicotiana tabacum L cv. Xanthi) plants that carried a fusion between the PI-Ⅱ promoter and the chloramphenicol acetyltransferase (cat) gene. PI-Ⅱ promoter activity was low when plants were young, but increased as plants grew. In 8-week-old plants, old leaves showed higher activity than young leaves. At flowering stage (ca. 15 weeks), the overall promoter activity was reduced to a lower level except in the petals. Compared with stems or petioles at the flowering stage, the roots and floral organs showed minimal activity for the PI-Ⅱ promoter. We used several environmental stimuli to examine the induction of the PI-Ⅱ promoter in different organs. Promoter induction was effected by wounding or methyl jasmonate in stems, petioles, sepals, and leaves. The induction was highest in leaves, as was sucrose-enhanced wound induction. These results suggest that the PI-Ⅱ gene is temporally and spatially regulated. We also established a transient assay system in tobacco BY2 suspension cells to elucidate the upstream regulatory region of the PI-Ⅱ promoter. A field strength of 0.75㎸/㎝ and 400㎌ capacitance were optimal electroporation conditions for our transient assay.
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